5GGY
X-ray crystal structure of Periplasmic Desferal binding protein FhuD from Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-09 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.980 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.246, 71.746, 125.889 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.316 - 2.500 |
R-factor | 0.1926 |
Rwork | 0.190 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1efd |
RMSD bond length | 0.003 |
RMSD bond angle | 0.729 |
Data scaling software | SCALA |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.320 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 18616 | |
<I/σ(I)> | 9.1 | |
Completeness [%] | 100.0 | |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18%(w/v) PEG 6000, 0.1 M MES (pH 6.0), 0.1M Tris (pH 7) |