5GGW

Crystal structure of Class C beta-lactamase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97960
Spacegroup name	C 2 2 21
Unit cell lengths	83.732, 182.088, 141.881
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.436 - 1.762
R-factor	0.2312
Rwork	0.230
R-free	0.25890
RMSD bond length	0.008
RMSD bond angle	1.148
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.760
Number of reflections	100735
<I/σ(I)>	21.9
Completeness [%]	94.7
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.5	295	Bis-Tris pH 5.5 Ammonium sulfate