5GGU

Crystal structure of tremelimumab Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	98.691, 103.211, 184.748
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.665 - 2.292
R-factor	0.1876
Rwork	0.184
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.222
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.290
Number of reflections	42405
<I/σ(I)>	28.1
Completeness [%]	99.8
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50 mM Tris, pH 8.5, 30% PEG4,000, 0.2 M ammonium chloride, 10 mM CaCl2