5GGQ

Crystal structure of Nivolumab Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-05
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	88.342, 88.342, 215.237
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.178 - 1.900
R-factor	0.1971
Rwork	0.196
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.152
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	39971
<I/σ(I)>	50.8
Completeness [%]	99.8
Redundancy	17.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M citric acid, pH 3.5, 2.0 M ammonium sulfate