5GGI

Crystal structure of human protein O-mannose beta-1,2-N-acetylglucosaminyltransferase in complex with Mn, UDP and Mannosyl-peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-21
Detector	RAYONIX MX300HE
Wavelength(s)	1.0000
Spacegroup name	P 2 21 21
Unit cell lengths	68.162, 126.539, 172.865
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.372 - 2.600
R-factor	0.2009
Rwork	0.199
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ggg
RMSD bond length	0.005
RMSD bond angle	0.784
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.370	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.338	1.085
Number of reflections	46907
<I/σ(I)>	20.5	2.1
Completeness [%]	100.0	100
Redundancy	26.2	11.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	NaKPO4