5GGG
Crystal structure of human protein O-mannose beta-1,2-N-acetylglucosaminyltransferase form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-31 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 99.917, 99.917, 236.104 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.684 - 3.000 |
| R-factor | 0.2072 |
| Rwork | 0.205 |
| R-free | 0.24120 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.947 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.684 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.067 | 0.657 |
| Number of reflections | 45699 | |
| <I/σ(I)> | 30.8 | 4.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.2 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | Na malonate |
