5GG6
Crystal structure of Mycobacterium smegmatis MutT1 in complex with 8-oxo-dGTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.95372 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.109, 89.500, 59.930 |
Unit cell angles | 90.00, 99.07, 90.00 |
Refinement procedure
Resolution | 49.360 - 1.750 |
R-factor | 0.16434 |
Rwork | 0.162 |
R-free | 0.20395 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fjy |
RMSD bond length | 0.019 |
RMSD bond angle | 1.968 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.360 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 59643 | |
<I/σ(I)> | 9.8 | 1.9 |
Completeness [%] | 99.4 | 98.8 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 8.5 | 293 | 0.2 M sodium acetate trihydrate, 0.1 M TRIS hydrochloride, 30% w/v PEG 4000 |