5GG5
Crystal structure of Mycobacterium smegmatis MutT1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.450, 87.660, 101.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.850 - 1.640 |
| R-factor | 0.15867 |
| Rwork | 0.157 |
| R-free | 0.19740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fjy |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.034 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.850 | 1.730 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Number of reflections | 41476 | |
| <I/σ(I)> | 11.4 | 3.6 |
| Completeness [%] | 99.4 | 96.3 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 293 | 0.2 M ammonium sulfate, 0.1 M MES monohydrate, 30% w/v PEG monomethyl ether 5000, 0.01M TCEP hydrochloride |
