5G5T
Structure of the Argonaute protein from Methanocaldcoccus janaschii in complex with guide DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-11 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 117.950, 117.950, 134.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.110 - 2.850 |
| R-factor | 0.2315 |
| Rwork | 0.229 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g5s |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.280 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.400 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.120 | 1.250 |
| Number of reflections | 22659 | |
| <I/σ(I)> | 7.8 | 1.1 |
| Completeness [%] | 1.0 | 99 |
| Redundancy | 5.4 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
