5G5R
CBS domain tandem of site-2 protease from Archaeoglobus fulgidus in complex with llama Nanobody - apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2013-11-30 |
| Detector | DECTRIS PLIATUS 2M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 68.440, 68.440, 196.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.979 - 2.400 |
| R-factor | 0.2093 |
| Rwork | 0.207 |
| R-free | 0.24980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 4FHB AND 3KPB |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.443 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.180 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.880 |
| Number of reflections | 11381 | |
| <I/σ(I)> | 24.24 | 2.31 |
| Completeness [%] | 99.7 | 97.9 |
| Redundancy | 9.16 | 8.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | WITH 0.14 M BIS-TRIS PH 6.0, 0.28 M (NH4)2SO4 AND 19% PEG 3350 |
