5G4Y
Structural basis for carboxylic acid recognition by a Cache chemosensory domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-11 |
| Detector | ADSC CCD |
| Spacegroup name | P 64 |
| Unit cell lengths | 74.282, 74.282, 75.057 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.330 - 2.000 |
| R-factor | 0.16196 |
| Rwork | 0.161 |
| R-free | 0.18315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k08 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.857 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.760 |
| Number of reflections | 16085 | |
| <I/σ(I)> | 14.2 | 2.9 |
| Completeness [%] | 99.8 | 97.8 |
| Redundancy | 11.2 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PROTEIN AT 8.5 MG/ML IN 50 MM BIS-TRIS CHLORIDE, PH 6.5, 50 MM SODIUM CHLORIDE IN EQUAL VOLUME WITH 27% PEG 4000 AND 100 MM PROLINE AT 20 C IN SITTING DROPS. |
