5G4U
Association of three two-k-turn units based on Kt-7 3bU,3nU, forming a triangular-shaped structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-20 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 261.260, 69.220, 85.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.870 - 2.650 |
| R-factor | 0.23 |
| Rwork | 0.229 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bw0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.129 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.250 | 2.720 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.100 | 1.500 |
| Number of reflections | 45710 | |
| <I/σ(I)> | 12.6 | 1 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 5.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.6 M TRI-SODIUM CITRATE DIHYDRATE (PH 6.5) |
