5G4T

The structure of a quasi-cyclic six k-turn duplex RNA species

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-02
Detector	DECTRIS PIXEL
Spacegroup name	P 63 2 2
Unit cell lengths	70.306, 70.306, 47.827
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.611 - 2.751
R-factor	0.1973
Rwork	0.195
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cs1
RMSD bond length	0.003
RMSD bond angle	0.630
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.330	2.850
High resolution limit [Å]	2.750	2.750
Rmerge	0.040	1.500
Number of reflections	2019
<I/σ(I)>	27.7	0.8
Completeness [%]	99.9	100
Redundancy	11.6	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		3.5 M NA FORMATE, 0.1 M NA ACETATE (PH 4.6)