5G4L
Phloroglucinol reductase from Clostridium sp. with bound NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 88.686, 123.083, 55.477 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.030 - 1.800 |
| R-factor | 0.1559 |
| Rwork | 0.155 |
| R-free | 0.17920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g4k |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.140 | 1.170 |
| Number of reflections | 57138 | |
| <I/σ(I)> | 17 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.2 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 28 % PEG 3350 0.1 M BIS-TRIS, PH 7.2 LI2SO4 3 MM NADPH |
