5G4K
Phloroglucinol reductase from Clostridium sp. apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 89.756, 125.391, 55.468 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.980 - 1.740 |
| R-factor | 0.1582 |
| Rwork | 0.157 |
| R-free | 0.18180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hq1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.980 | 1.830 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.110 | 0.870 |
| Number of reflections | 65090 | |
| <I/σ(I)> | 13.7 | 2.4 |
| Completeness [%] | 99.3 | 99.4 |
| Redundancy | 8.2 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.75 | 31% PEG 3350 0.1 M BIS-TRIS, PH 5.75 LI2SO4 |
