5G3X

Structure of recombinant granulovirus polyhedrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-05
Detector	MARRESEARCH
Spacegroup name	I 2 3
Unit cell lengths	102.058, 102.058, 102.058
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.276 - 1.660
R-factor	0.1535
Rwork	0.149
R-free	0.19220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jvb
RMSD bond length	0.007
RMSD bond angle	0.862
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.280	1.720
High resolution limit [Å]	1.660	1.660
Rmerge	0.260
Number of reflections	21009
<I/σ(I)>	7.5	1.5
Completeness [%]	99.9	99.7
Redundancy	8.7	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		IN VIVO CRYSTALLIZATION, pH 7.0