5G36
Yellow form of Halorhodopsin from Halobacterium salinarum in a new rhombohedral crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-05 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | H 3 2 |
Unit cell lengths | 103.000, 103.000, 136.450 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.393 - 2.600 |
R-factor | 0.1943 |
Rwork | 0.191 |
R-free | 0.25270 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 0.999 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.500 | 2.730 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.160 | 0.910 |
Number of reflections | 8774 | |
<I/σ(I)> | 14.5 | 1.8 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 8.2 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 296 | RESERVOIR SOLUTION: 100 MM BICINE PH 8, 150 MM NACL, 2.4 M (NH4)2SO4. VAPOR DIFFUSION AT 296 K WITH DROP RATIO OF 1.2 TO 0.8 UL PROTEIN TO RESERVOIR. PROTEIN CONCENTRATION: 4.9 MG/ML. POST-CRYSTALLIZATION SOAKING SOLUTION: 100 MM GLYCINE PH 10, 2.6 M (NH4)2SO4 PH 10, 25% SUCROSE. |