5G33
Structure of Rad14 in complex with acetylnaphtyl-guanine containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-18 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 41 |
| Unit cell lengths | 53.369, 53.369, 131.252 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.370 - 2.400 |
| R-factor | 0.25341 |
| Rwork | 0.251 |
| R-free | 0.29832 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a3d |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.603 |
| Data reduction software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.400 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.190 | 1.390 |
| Number of reflections | 14071 | |
| <I/σ(I)> | 5.8 | 1.1 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
