5G28
The crystal structure of light-driven chloride pump ClR at pH 6.0.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2015-07-27 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.756, 49.402, 69.327 |
| Unit cell angles | 90.00, 109.85, 90.00 |
Refinement procedure
| Resolution | 65.210 - 1.570 |
| R-factor | 0.15594 |
| Rwork | 0.154 |
| R-free | 0.19378 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3x3b |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.904 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.210 | 1.590 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.110 | 0.660 |
| Number of reflections | 45878 | |
| <I/σ(I)> | 30.81 | 2.58 |
| Completeness [%] | 97.2 | 90.7 |
| Redundancy | 4.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | pH 6.0 |
