5G1O
Aspartate transcarbamoylase domain of human CAD in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.790, 157.890, 82.720 |
| Unit cell angles | 90.00, 120.00, 90.00 |
Refinement procedure
| Resolution | 42.430 - 2.100 |
| R-factor | 0.15153 |
| Rwork | 0.150 |
| R-free | 0.17172 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3csu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.284 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.400 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.120 | 0.610 |
| Number of reflections | 95831 | |
| <I/σ(I)> | 8.9 | 2.4 |
| Completeness [%] | 94.3 | 63.9 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS PH 8.5, 6% PEG 8000, 11% ETHYLENE GLYCOL |
