5G1M
Crystal structure of NagZ from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.971, 75.289, 76.389 |
| Unit cell angles | 90.00, 110.10, 90.00 |
Refinement procedure
| Resolution | 46.959 - 1.800 |
| R-factor | 0.1979 |
| Rwork | 0.196 |
| R-free | 0.24060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oxn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.064 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.960 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.110 | 0.940 |
| Number of reflections | 63078 | |
| <I/σ(I)> | 9.5 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 30% PEG 8000 100 MM SODIUM CACODYLATE PH 6.0 200 MM SODIUM ACETATE PH 6.0 |
