5G13
Pseudomonas aeruginosa HDAH (H143A) unliganded.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 81.495, 81.495, 205.265 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.740 - 1.990 |
| R-factor | 0.17484 |
| Rwork | 0.173 |
| R-free | 0.21723 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g0x |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.323 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.740 | 2.050 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.180 | 0.340 |
| Number of reflections | 48272 | |
| <I/σ(I)> | 11.2 | 4.8 |
| Completeness [%] | 99.5 | 95.9 |
| Redundancy | 15.5 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
