5FZT
The crystal structure of R7R8 in complex with a DLC1 fragment.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-20 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 73.260, 73.260, 111.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.181 - 2.100 |
| R-factor | 0.1794 |
| Rwork | 0.177 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x0c |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.885 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10 (PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.180 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.130 | 0.650 |
| Number of reflections | 20847 | |
| <I/σ(I)> | 10.11 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 15% ETHANOL, 0.1 M TRIS PH 7.4 AT 4OC |
