5FZT
The crystal structure of R7R8 in complex with a DLC1 fragment.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-20 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 73.260, 73.260, 111.820 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 55.181 - 2.100 |
R-factor | 0.1794 |
Rwork | 0.177 |
R-free | 0.23060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x0c |
RMSD bond length | 0.008 |
RMSD bond angle | 0.885 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.10 (PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.180 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.130 | 0.650 |
Number of reflections | 20847 | |
<I/σ(I)> | 10.11 | 2.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.2 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 15% ETHANOL, 0.1 M TRIS PH 7.4 AT 4OC |