5FYA
Cubic crystal of the native PlpD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 145.906, 145.906, 145.906 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.635 - 2.141 |
| R-factor | 0.2013 |
| Rwork | 0.199 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fqu |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.787 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.640 | 2.270 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 54378 | |
| <I/σ(I)> | 61.6 | 3 |
| Completeness [%] | 95.3 | 85.4 |
| Redundancy | 5.9 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.4 M MAGNESIUM SULFATE, 0.1M BIS-TRIS PROPANE PH 7.0 |
