5FY7
Crystal structure of JmjC domain of human histone demethylase UTY in complex with succinate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-23 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.690, 110.770, 119.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.385 - 1.860 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zli |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | DIMPLE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.380 | 1.910 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.070 | 1.380 |
| Number of reflections | 102989 | |
| <I/σ(I)> | 15.9 | 1.1 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 0.1M HEPES PH 7.2, 25% PEG3350 |
