5FY5
Crystal structure of the catalytic domain of human JARID1B in complex with fumarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-20 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 141.680, 141.680, 153.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.284 - 2.470 |
| R-factor | 0.1922 |
| Rwork | 0.190 |
| R-free | 0.22990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a3p |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.227 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | DIMPLE |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1682)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.280 | 2.530 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.200 | 1.500 |
| Number of reflections | 33120 | |
| <I/σ(I)> | 10.3 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.8 | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PH 7.0 |
