5FXX

Crystal structure of JmjC domain of human histone demethylase UTY in complex with oxaloacetate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-23
Detector	DECTRIS PILATUS 2M
Spacegroup name	P 21 21 21
Unit cell lengths	91.590, 110.700, 119.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.773 - 1.990
R-factor	0.1955
Rwork	0.193
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	DPB ENTRY 3ZLI
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	DIMPLE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.770	2.040
High resolution limit [Å]	1.990	1.990
Rmerge	0.120	1.460
Number of reflections	83981
<I/σ(I)>	10.8	1.2
Completeness [%]	99.9	99.9
Redundancy	6.7	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.2		0.1M HEPES PH 7.2 -- 25% PEG3350