5FXV
Crystal structure of JmjC domain of human histone demethylase UTY in complex with N05859b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.650, 109.910, 117.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.902 - 1.910 |
| R-factor | 0.2007 |
| Rwork | 0.199 |
| R-free | 0.23990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zli |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | DIMPLE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 1.960 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.120 | 1.500 |
| Number of reflections | 91818 | |
| <I/σ(I)> | 9.9 | 1.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 0.1M HEPES PH 7.2 -- 25% PEG3350 |
