5FWZ
Fasciola hepatica calcium binding protein FhCaBP2: Structure of the dynein light chain-like domain. P41212 mercury derivative.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.880, 59.880, 81.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.830 - 2.300 |
| R-factor | 0.1965 |
| Rwork | 0.191 |
| R-free | 0.24051 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.469 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.120 | 0.470 |
| Number of reflections | 7026 | |
| <I/σ(I)> | 12 | 4.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.6 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 10 MM TRIS-HCL PH 8.5, 50 MM SODIUM CHLORIDE 20 % (W/V) PEG 3350, 0.2 M SODIUM TARTRATE |
