5FWY
Crystal structure of the AMPA receptor GluA2/A3 N-terminal domain heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-09 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.270, 78.519, 107.850 |
| Unit cell angles | 83.36, 87.40, 64.94 |
Refinement procedure
| Resolution | 41.760 - 2.120 |
| R-factor | 0.19418 |
| Rwork | 0.192 |
| R-free | 0.23113 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3HSY AND 3O21 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.734 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.750 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.100 | 0.440 |
| Number of reflections | 100269 | |
| <I/σ(I)> | 6 | 1.9 |
| Completeness [%] | 90.4 | 87.8 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 14-16 % PEG 3350, 0.27 M AMMONIUM SULPHATE AND 0.1 M BICINE PH 9 |
