5FWX
Crystal structure of the AMPA receptor GluA2/A4 N-terminal domain heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-09 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.070, 156.630, 77.120 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 39.160 - 2.500 |
| R-factor | 0.17507 |
| Rwork | 0.172 |
| R-free | 0.23816 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 4GPA AND 3HSY |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.624 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.680 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.350 |
| Number of reflections | 45771 | |
| <I/σ(I)> | 12.2 | 3 |
| Completeness [%] | 96.3 | 96 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 17% PEG 3350 WITH 0.1-0.35 M AMMONIUM SULPHATE |
