5FWW
Wnt modulator Kremen in complex with DKK1 (CRD2) and LRP6 (PE3PE4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-15 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 86.933, 100.080, 270.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 135.360 - 3.500 |
| R-factor | 0.32284 |
| Rwork | 0.321 |
| R-free | 0.35528 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.998 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 67.680 |
| High resolution limit [Å] | 3.500 |
| Rmerge | 0.370 |
| Number of reflections | 8070 |
| <I/σ(I)> | 4.6 |
| Completeness [%] | 51.6 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20 %W/V PEG3350 0.2 M NA/K-PHOSPHATE, pH 7.5 |
