5FWU
Wnt modulator Kremen crystal form II at 2.8A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-15 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 43 |
| Unit cell lengths | 65.780, 65.780, 75.014 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.780 - 2.800 |
| R-factor | 0.22003 |
| Rwork | 0.216 |
| R-free | 0.30707 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.952 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 75.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.060 |
| Number of reflections | 7964 |
| <I/σ(I)> | 14.9 |
| Completeness [%] | 99.8 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 0.1 M NA3CITRATE, 1.0 M MGSO4, FINAL PH 5.6 |
