5FWG
TETRA-(5-FLUOROTRYPTOPHANYL)-GLUTATHIONE TRANSFERASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-08 |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.939, 68.747, 80.539 |
| Unit cell angles | 90.00, 105.08, 90.00 |
Refinement procedure
| Resolution | 65.000 - 2.000 |
| R-factor | 0.181 * |
| Structure solution method | MOLECULAR MODIFICATION |
| Starting model (for MR) | 3gst |
| RMSD bond length | 0.022 * |
| RMSD bond angle | 3.030 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.137 |
| Total number of observations | 184888 * | |
| Number of reflections | 29587 * | |
| <I/σ(I)> | 20 | 15 |
| Completeness [%] | 95.0 * | 68.3 |
| Redundancy | 6.24 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.6 | 22 * | pH 7.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | inhibitor | 2 (mM) | |
| 3 | 1 | drop | n-octyl-beta-D-glycopyranoside | 0.3 (%) | |
| 4 | 1 | drop | PEG3000 | 10 (%) | |
| 5 | 1 | drop | HEPES | 100 (mM) | |
| 6 | 1 | reservoir | PEG3000 | 24 (%) |
