5FWA

Crystal Structure of Mus musculus Protein Arginine Methyltransferase 2 with CP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-25
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 2 2 21
Unit cell lengths	66.183, 114.676, 132.653
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.369 - 1.800
R-factor	0.1727
Rwork	0.172
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fub
RMSD bond length	0.006
RMSD bond angle	0.854
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.200	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.090
Number of reflections	47105
<I/σ(I)>	11.7	1
Completeness [%]	100.0	100
Redundancy	6.5	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		24% PEG6000, 100 MM HEPES PH7, 100MM CACL2