5FVK
Crystal structure of Vps4-Vfa1 complex from S.cerevisiae at 1.66 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.830, 56.460, 84.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.994 - 1.658 |
| R-factor | 0.1857 |
| Rwork | 0.184 |
| R-free | 0.22230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v6x |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.938 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.990 | 1.750 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.100 | 0.610 |
| Number of reflections | 22657 | |
| <I/σ(I)> | 16.1 | 4.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.4 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | pH 4 |
