5FVD
Human metapneumovirus N0-P complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.930, 62.780, 86.710 |
| Unit cell angles | 90.97, 96.38, 108.98 |
Refinement procedure
| Resolution | 28.421 - 1.860 |
| R-factor | 0.1729 |
| Rwork | 0.171 |
| R-free | 0.20530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wj8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.951 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.420 | 1.910 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.060 | 0.590 |
| Number of reflections | 64451 | |
| <I/σ(I)> | 9.2 | 1.1 |
| Completeness [%] | 94.8 | 75 |
| Redundancy | 1.7 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 100 MM PCB SYSTEM, PH 7, 25 % POLYETHYLENE GLYCOL 1500, 9 MM 1,2-DIAMINOCYCLOHEXANE SULFATE, 6 MM DILOXANIDE FUROATE, 17 MM FUMARIC ACID, 10 MM SPERMINE, 9 MM SULFAGUANIDINE AND 20 MM HEPES, PH 6.8 (SILVER BULLETS ADDITIVES) |
