5FVC
Structure of RNA-bound decameric HMPV nucleoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 202.010, 233.210, 203.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 101.190 - 4.170 |
R-factor | 0.1932 |
Rwork | 0.191 |
R-free | 0.23020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | DECAMER OF MONOMERIC HMPV N0 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.120 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 101.190 | 4.280 |
High resolution limit [Å] | 4.170 | 4.170 |
Rmerge | 0.220 | |
Number of reflections | 36125 | |
<I/σ(I)> | 9.2 | 1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 13.5 | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 100 MM TRIS/BICINE, PH 8.5, 90 MM NPS (NAN03, NA2HPO4, (NH4)2SO4), 37.5 % METHYL-2 4-PENTANEDIOL, POLYETHYLENE GLYCOL 1000 AND POLYETHYLENE GLYCOL 3350 (MORPHEUS SCREEN) |