5FV5
KpFlo11 presents a novel member of the Flo11 family with a unique recognition pattern for homophilic interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.540, 58.520, 76.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.400 |
| R-factor | 0.13596 |
| Rwork | 0.134 |
| R-free | 0.17937 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uys |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.659 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 42777 | |
| <I/σ(I)> | 20.95 | 4.7 |
| Completeness [%] | 95.9 | 94.7 |
| Redundancy | 6.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.2 M AMMONIUM ACETATE, 0.1 M SODIUM ACETATE PH 4.6, 15 % PEG 4000 |
