5FV2
Crystal structure of hVEGF in complex with VH domain antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Temperature [K] | 100 |
| Collection date | 2009-06-19 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.108, 130.397, 81.178 |
| Unit cell angles | 90.00, 106.53, 90.00 |
Refinement procedure
| Resolution | 39.620 - 3.450 |
| R-factor | 0.21484 |
| Rwork | 0.212 |
| R-free | 0.26270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.789 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.340 | 3.640 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.010 | 0.440 |
| Number of reflections | 14048 | |
| <I/σ(I)> | 7.8 | 2.7 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 293 | 0.1M TRIS PH 8.5, 7%PEG6000, 0.2M MGCL2 20C |
