5FUI
Crystal structure of the C-terminal CBM6 of LamC a marine laminarianse from Zobellia galactanivorans
Replaces: 4CRRExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-08 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.056, 29.523, 51.872 |
| Unit cell angles | 90.00, 115.79, 90.00 |
Refinement procedure
| Resolution | 46.710 - 1.400 |
| R-factor | 0.12787 |
| Rwork | 0.126 |
| R-free | 0.12704 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uxz |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.064 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.700 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.060 | 0.360 |
| Number of reflections | 19499 | |
| <I/σ(I)> | 10.5 | 2 |
| Completeness [%] | 93.0 | 65.3 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 100 MM HEPES PH 7.2, 10 MM MGCL2 AND 27 % POLYACRYLIC ACID. MICROSEEDING WAS ESSENTIAL |
