5FT5

Crystal structure of the cysteine desulfurase CsdA (persulfurated) from Escherichia coli at 2.384 Angstroem resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	58.347, 99.533, 141.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.721 - 2.384
R-factor	0.1697
Rwork	0.167
R-free	0.22450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ft4
RMSD bond length	0.004
RMSD bond angle	0.774
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AMoRE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.720	2.470
High resolution limit [Å]	2.380	2.380
Rmerge	0.180	0.740
Number of reflections	29983
<I/σ(I)>	11.1	2.4
Completeness [%]	89.4	91.5
Redundancy	4.1	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1