5FT3
Aedes aegypti GSTe2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-28 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.811, 86.563, 101.021 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.745 - 1.431 |
R-factor | 0.1359 |
Rwork | 0.134 |
R-free | 0.17400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zml |
RMSD bond length | 0.005 |
RMSD bond angle | 0.960 |
Data reduction software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.850 | 1.470 |
High resolution limit [Å] | 1.430 | 1.430 |
Rmerge | 0.060 | 0.590 |
Number of reflections | 78165 | |
<I/σ(I)> | 18.8 | 2.1 |
Completeness [%] | 98.0 | 80.7 |
Redundancy | 5.7 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2 M NACL, 0.1 M BIS-TRIS PH 5.7, 25 % PEG 3350 |