5FS7
Crystal structure of the G262S mutant of human apoptosis inducing factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2015-09-12 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 91.410, 114.570, 120.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 114.530 - 1.850 |
R-factor | 0.18072 |
Rwork | 0.179 |
R-free | 0.21691 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fs8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.444 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.130 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.120 | 1.040 |
Number of reflections | 106911 | |
<I/σ(I)> | 9.2 | 1.5 |
Completeness [%] | 98.6 | 97.4 |
Redundancy | 5.5 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 0.2 M LITHIUM SULFATE, 0.1M TRIS, PH 8.5, AND 25% POLY-ETHYLENE GLYCOL 3350 |