5FS0

crystal structure of the phenol-responsive sensory domain of the transcription activator PoxR with 2,4-dichlorophenol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	287
Spacegroup name	P 21 21 21
Unit cell lengths	46.693, 70.872, 104.852
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.346 - 2.400
R-factor	0.1854
Rwork	0.181
R-free	0.25800
Structure solution method	MIR
Starting model (for MR)	NONE
RMSD bond length	0.007
RMSD bond angle	0.990
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.820
High resolution limit [Å]	1.790	1.790
Rmerge	0.080
Number of reflections	33443
<I/σ(I)>	49.41	1
Completeness [%]	99.2	89.6
Redundancy	13.7	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1