5FRY

crystal structure of the phenol-responsive sensory domain of the transcription activator PoxR with 3,5-dimethylphenol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	287
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	46.730, 70.696, 105.141
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.191 - 1.792
R-factor	0.268
Rwork	0.265
R-free	0.31990
Structure solution method	MIR
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	1.079
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.191	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.080	0.680
Number of reflections	33213
<I/σ(I)>	55.11	2.33
Completeness [%]	99.4	99
Redundancy	13.8	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1