5FRE
Characterization of a novel CBM from Clostridium perfringens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-28 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.348, 76.714, 67.254 |
| Unit cell angles | 90.00, 93.46, 90.00 |
Refinement procedure
| Resolution | 40.610 - 1.900 |
| R-factor | 0.17471 |
| Rwork | 0.173 |
| R-free | 0.20874 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fra |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.718 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.690 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.390 |
| Number of reflections | 50882 | |
| <I/σ(I)> | 13.2 | 3 |
| Completeness [%] | 98.8 | 93.4 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 0.1M CH3COONA PH 4.5, 0.2M (CH3CHOO)2CA, 8% (PEG 550 MME AND PEG 20,000) |
