5FPK
MONOMERIC RADA IN COMPLEX WITH FATA TETRAPEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.302, 60.578, 87.447 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.723 - 1.343 |
| R-factor | 0.1251 |
| Rwork | 0.123 |
| R-free | 0.16370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b3b |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.033 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.800 | 1.420 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.070 | 0.610 |
| Number of reflections | 47749 | |
| <I/σ(I)> | 22.41 | 2.98 |
| Completeness [%] | 98.3 | 92.3 |
| Redundancy | 6.98 | 6.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 8% PEG-1000, 100 MM NAK PHOSPHATE, PH 6.2 |
