5FOK
Crystal structure of the siderophore receptor PiuA from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-10 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 194.010, 132.970, 73.590 |
| Unit cell angles | 90.00, 102.21, 90.00 |
Refinement procedure
| Resolution | 71.930 - 1.900 |
| R-factor | 0.18955 |
| Rwork | 0.188 |
| R-free | 0.21058 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fcp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.217 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.930 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.730 |
| Number of reflections | 142771 | |
| <I/σ(I)> | 13.7 | 2.5 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 32% PEG 400, 0.05 M TRIS PH 8.5, 0.05 M SODIUM SULPHATE AND 0.05 M LICL2 |
