5FOI
Crystal structure of mycinamicin VIII C21 methyl hydroxylase MycCI from Micromonospora griseorubida bound to mycinamicin VIII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-09-03 |
| Detector | MARRESERCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.781, 59.516, 74.623 |
| Unit cell angles | 83.20, 72.15, 62.61 |
Refinement procedure
| Resolution | 71.000 - 2.210 |
| R-factor | 0.18572 |
| Rwork | 0.182 |
| R-free | 0.25150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cv9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.907 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.000 | 2.330 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.240 | 1.470 |
| Number of reflections | 36326 | |
| <I/σ(I)> | 3.8 | 1.3 |
| Completeness [%] | 91.1 | 71.2 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20% PEG 3350, 0.2 M CA ACETATE, 20 MM SPERMIDINE, 0.84 MM TCEP, pH 7 |
